Dic Transmitted Light Microscopy

A polarizer placed just after the light source creates plane polarized light that is passed through a wollaston prism which splits the beam into two beams that have their axis of vibration at 90 relative to each other.

Dic transmitted light microscopy. In this way it is possible to observe living cells and organisms with adequate contrast and resolution. Differential interference contrast. Differential interference contrast dic microscopy also known as nomarski interference contrast nic or nomarski microscopy is an optical microscopy technique used to enhance the contrast in unstained transparent samples dic works on the principle of interferometry to gain information about the optical path length of the sample to see otherwise invisible features. Dic microscopy is a technique which uses gradients in the optical path length and phase shifts to make phase objects visible under the light microscope.

Differential interference contrast reflected light dic microscopy. When compared to the typical configuration employed in transmitted light microscopy the critical instrument parameters for reflected or episcopic light differential interference contrast dic are much simpler primarily because only a single birefringent nomarski or wollaston prism is required and the objective serves as both the condenser and image forming optical system. With dic microscopy two slightly separate plane polarized beams of light are used to create a 3d like image of the unstained specimen.